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A microarray enzyme‐linked immunosorbent assay for autoimmune diagnostics
Author(s) -
Joos Thomas O.,
Schrenk Monika,
Höpfl Peter,
Kröger Kerstin,
Chowdhury Ushashi,
Stoll Dieter,
Schörner Dominik,
Dürr Manfred,
Herick Klaus,
Rupp Steffen,
Sohn Kai,
Hämmerle Hugo
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(20000701)21:13<2641::aid-elps2641>3.0.co;2-5
Subject(s) - serial dilution , immunoassay , autoantibody , protein microarray , microarray , titer , antigen , autoimmune disease , protein array analysis , antibody , chemistry , immunology , microbiology and biotechnology , dna microarray , biology , medicine , biochemistry , gene , gene expression , pathology , alternative medicine
In order to quantify autoantibodies in the sera of patients with autoimmune disease, we have created a microarray‐based immunoassay that allows the simultaneous analysis of 18 known autoantigens. The microarrays contain serial dilutions of the various antigens, thereby allowing accurate determination of autoantibody titer using minimal amounts of serum. The assay is very sensitive and highly specific: as little as 40 fg of a known protein standard can be detected with little or no cross‐reactivity to nonspecific proteins. The signal intensities observed from serial dilutions of immobilized antigen correlate well with serial dilutions of autoimmune sera. Miniaturized and highly parallelized immunoassays like these will reduce costs by decreasing reagent consumption and improve efficiency by greatly increasing the number of assays that can be performed with a single serum sample. This system will significantly facilitate and accelerate the diagnostics of autoimmune diseases and can be adapted easily to any other kind of immunoassay.