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Towards high performance two‐dimensional gel electrophoresis using ultrazoom gels
Author(s) -
Hoving Sjouke,
Voshol Hans,
van Oostrum Jan
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(20000701)21:13<2617::aid-elps2617>3.0.co;2-c
Subject(s) - proteome , gel electrophoresis , two dimensional gel electrophoresis , proteomics , electrophoresis , chromatography , gel electrophoresis of proteins , high resolution , chemistry , polyacrylamide gel electrophoresis , biochemistry , enzyme , remote sensing , gene , geology
Proteomics is the analysis of protein expression in cells or tissues, e.g. , to study cellular processes at the molecular level. Ultimately, a proteome analysis should encompass most if not all protein species in a biological sample, including those present in low copy numbers. We are developing two‐dimensional gel electrophoresis technology by applying narrow pH range ultrazoom gels to enhance resolution and to improve the detection of low abundance proteins. Ultrazoom gels in the acidic pH range allow the detection of proteins down to 300 copies per cell of a B‐lymphoma cell line. Protein separation in the alkaline pH range, however, still requires optimization, especially in conjunction with high sample loads.