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Capillary electrophoresis separation of vinpocetine and related compounds: Prediction of electrophoretic mobilities in partly aqueous media
Author(s) -
Mazák Károly,
Szakács Zoltán,
Nemes Andreás,
Noszál Béla
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(20000701)21:12<2417::aid-elps2417>3.0.co;2-p
Subject(s) - electrophoresis , capillary electrophoresis , chemistry , chromatography , aqueous solution , analytical chemistry (journal) , vinpocetine , solubility , organic chemistry , medicine , pharmacology
Offord's equation, a relationship between electrophoretic mobility and charge, size and shape of peptides, has been extended to quantitate the electrophoretic mobility of vinca alkaloids. Partly aqueous protonation constants and the derived theoretical mobilities have been proven to be able to predict experimental electrophoretic mobilies. In practice, seven vincamine derivatives of very low water‐solubility were separated by capillary electrophoresis. Buffer total concentration, apparent pH and methanol content, the three most important parameters of the running buffer, were used in triangular resolution mapping to characterize separation. Even though electrophoresis is well known to slow down in partly aqueous media, under our optimized circumstances a baseline separation was achieved within 8 min in each case.