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Development of a capillary zone electrophoresis method for the separation of a furan combinatorial library
Author(s) -
Lee Doowon,
Shamsi Shahab A.
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(20000701)21:12<2405::aid-elps2405>3.0.co;2-2
Subject(s) - capillary electrophoresis , chromatography , chemistry , buffer (optical fiber) , electrophoresis , furan , electrolyte , acetonitrile , resolution (logic) , phosphate buffered saline , buffer solution , phosphate , theoretical plate , analytical chemistry (journal) , organic chemistry , electrode , telecommunications , artificial intelligence , computer science
Nine component mixtures of a furan library were simultaneously separated by capillary zone electrophoresis (CZE) using a phosphate buffer as a background electrolyte at low pH. The effects of buffer concentration, buffer pH, type and concentration of organic solvents on the electrophoretic mobility, resolution, and analysis time were systematically investigated. Resolution and efficiency of furan library components were further improved using cyclodextrin (CD)‐modified CZE. Under optimum conditions, eight of the nine furans were baseline‐resolved in less than 10 min at 30 kV using 50 m M phosphate buffer, 10% v/v acetonitrile (ACN), pH 2.0, with 5 m M γ‐CD.