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Solid‐state UV laser‐induced fluorescence detection in capillary electrophoresis
Author(s) -
Chan King C.,
Muschik Gary M.,
Issaq Haleem J.
Publication year - 2000
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683(20000601)21:10<2062::aid-elps2062>3.0.co;2-z
Subject(s) - fluorescamine , capillary electrophoresis , laser induced fluorescence , fluorescence , chemistry , laser , chromatography , derivatization , tryptophan , fluorescence spectroscopy , detection limit , electrophoresis , analytical chemistry (journal) , mass spectrometry , amino acid , optics , biochemistry , physics
Two solid‐state UV lasers were applied to the laser‐induced fluorescence (LIF) detection of various groups of compounds after separation by capillary electrophoresis. These lasers are thermoelectric‐cooled, highly compact, and inexpensive. Such lasers provide few mW of quasi‐continuous wave (CW) power which are sufficient and stable for LIF detection. Native fluorescence detection of tryptophan‐containing proteins and peptides and related indoles was achieved at the n M level with the laser operating at 266 nm. Detection of fluorescamine‐labeled amino acids and peptides was also possible at the n M level with the laser operating at 355 nm. Amino acids at a concentration as low as 10 ng/mL could be labeled with fluorescamine. Solid‐state UV‐LIF detection of the tryptic digest of cytochrome c after fluorescamine derivatization was demonstrated.