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A two‐dimensional protein map of Caenorhabditis elegans
Author(s) -
Schrimpf Sabine P.,
Langen Hanno,
Gomes Ana Vaz,
Wahlestedt Claes
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683()22:6<1224::aid-elps1224>3.0.co;2-i
Subject(s) - computational biology , chemistry , biology
A protein map of Caenorhabditis elegans was constructed by using two‐dimensional gel electrophoresis followed by peptide mass fingerprinting. A whole worm extract of a mixed population was separated on immobilized pH gradient strips 4‐7 L, 3‐10 NL, 6‐11 L and 12 % sodium dodecyl sulfate‐polyacrylamide gel eletrophoresis (SDS‐PAGE) gels. Gels were stained with colloidal Coomassie blue and 286 spots representing 152 proteins were subsequently identified by matrix‐assisted laser desorption/ionization‐mass spectrometry after in‐gel digestion with trypsin. Most of the identified proteins with known cellular function were enzymes related to carbohydrate and lipid metabolism, or structural proteins with subcellular locations in the cytoplasm, mitochondria or cytoskeleton.