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Medium‐throughput p K a screening of pharmaceuticals by pressure‐assisted capillary electrophoresis
Author(s) -
Jia Zhongjiang,
Ramstad Tore,
Zhong Min
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683()22:6<1112::aid-elps1112>3.0.co;2-1
Subject(s) - capillary electrophoresis , electrophoresis , chemistry , chromatography , dissociation (chemistry) , analytical chemistry (journal) , dissociation constant , atmospheric pressure , throughput , organic chemistry , computer science , telecommunications , biochemistry , oceanography , receptor , wireless , geology
A fast screening method for the determination of the dissociation constants (p K a ) of acidic, basic, and multivalent compounds was developed by using pressure‐assisted capillary electrophoresis (PACE). External air pressure was applied to shorten the analysis time. The separation efficiency decreases as air pressure increases. However, it was found that air pressure does not affect the measurement of electrophoretic mobility and p K a significantly when it is less than 2 psi. The method was evaluated in terms of accuracy, precision, and ruggedness by using a set of 48 compounds with literature p K a values ranging from 2 to 10. The difference between the measured p K a values and literature values is less than 0.2 units. The throughput is approximately 20 compounds per day with a 12‐point measurement ranging from pH 2.5 to 11. It was demonstrated that this method is applicable for p K a screening of pharmaceuticals with diverse chemical structures.