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Detection of Saccharomyces cerevisiae carboxylesterase activity after native and sodium dodecyl sulfate electrophoresis by using fluorescein diacetate as substrate
Author(s) -
Lomolino Giovanna,
Lante Anna,
Crapisi Antonella,
Spettoli Paolo,
Curioni Andrea
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683()22:6<1021::aid-elps1021>3.0.co;2-b
Subject(s) - carboxylesterase , sodium dodecyl sulfate , esterase , chemistry , chromatography , substrate (aquarium) , zymography , fluorescein , fluorescence , electrophoresis , gel electrophoresis , sodium , biochemistry , enzyme , organic chemistry , biology , ecology , physics , quantum mechanics
A simple method for the visualisation of wine yeast esterase (carboxylesterase EC 3.1.1.1) activity on electrophoretic gels was developed, using the fluorescent substrate fluorescein diacetate. The zymogram system allows a sensitive detection of esterase bands in only 5 min of incubation of both native and sodium dodecyl sulfate gels.