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Quantifying DNA damage by gel electrophoresis, electronic imaging and number‐average length analysis
Author(s) -
Sutherland John C.,
Monteleone Denise C.,
Trunk John G.,
Bennett Paula V.,
Sutherland Betsy M.
Publication year - 2001
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/1522-2683()22:5<843::aid-elps843>3.0.co;2-9
Subject(s) - dna , gel electrophoresis , electrophoresis , gel electrophoresis of nucleic acids , fluorophore , charge coupled device , double strand , dna damage , chemistry , chromatography , microbiology and biotechnology , fluorescence , biology , optics , physics , biochemistry
Abstract DNA damages that can be converted to single‐ or double strand breaks can be quantified by separating DNA by gel electrophoresis and obtaining a quantitative image of the resulting distribution of DNA in the gel. We review the theory of this method and discuss its implementation, including the charge‐coupled device (CCD) camera systems we developed to acquire images of fluorophore labeled DNA.