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The Detection and Stability of DNA Duplexes Probed by MALDI Mass Spectrometry
Author(s) -
Sudha Rajagopalan,
Zenobi Renato
Publication year - 2002
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/1522-2675(200210)85:10<3136::aid-hlca3136>3.0.co;2-f
Subject(s) - chemistry , dna , mass spectrometry , duplex (building) , matrix assisted laser desorption/ionization , desorption , adduct , maldi imaging , ionization , ion , monomer , chromatography , analytical chemistry (journal) , polymer , biochemistry , organic chemistry , adsorption
Abstract Matrix‐assisted laser desorption/ionization (MALDI) with nonacidic matrices is shown to generate intact gas‐phase ions of double‐stranded DNA. Control experiments show that specific DNA duplexes are detected by this method, while nonspecific adducts form only when high monomer concentrations are present in the MALDI sample. The relative intensity of the duplex‐ion signal is found to reflect the solution‐phase stability of the double‐stranded DNA.