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Optimization Procedure, Applying the Experimental‐Design Methodology, for the Determination of Rifampicin after Metal Complexation by Differential Pulse Adsorptive Stripping Voltammetry
Author(s) -
Alonso Lomillo M. Asunción,
Domínguez Renedo Olga,
Arcos Martínez M. Julia
Publication year - 2002
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/1522-2675(200208)85:8<2430::aid-hlca2430>3.0.co;2-#
Subject(s) - chemistry , voltammetry , stripping (fiber) , adsorptive stripping voltammetry , differential pulse voltammetry , differential (mechanical device) , pulse (music) , rifampicin , anodic stripping voltammetry , chromatography , analytical chemistry (journal) , cyclic voltammetry , electrode , electrochemistry , thermodynamics , antibiotics , biochemistry , physics , detector , electrical engineering , engineering
A procedure for the determination of rifampicin (RIF) after Cu II complexation by differential pulse adsorptive stripping voltammetry (DPAdSV) was optimized. The selection of the experimental conditions was made on the basis of the experimental‐design methodology. The calibrations were performed under these conditions by means of a robust regression method that allows for the elimination of anomalous points. Once the detection limit was calculated, this method was successfully applied to the analysis of RIF in pharmaceutical preparations. The analysis of urine samples was also performed. The complexity of these samples made it necessary to apply multivariate‐regression techniques to obtain satisfactory results.