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Structural Properties Governing Retention Mechanisms on Immobilized Artificial Membrane (IAM) HPLC Columns
Author(s) -
TaillardatBertschinger Agnes,
Barbato Francesco,
Quercia Maria Tiziana,
Carrupt PierreAlain,
Reist Marianne,
La Rotonda Maria I.,
Testa Bernard
Publication year - 2002
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/1522-2675(200202)85:2<519::aid-hlca519>3.0.co;2-q
Subject(s) - chemistry , partition coefficient , solvation , chromatography , high performance liquid chromatography , hydrogen bond , solvatochromism , octanol , intermolecular force , dissociation (chemistry) , membrane , yield (engineering) , molecule , organic chemistry , thermodynamics , biochemistry , physics
The aims of this study were to investigate whether three commercially available immobilized artificial membrane (IAM) HPLC columns yield collinear data for neutral compounds, and whether IAM scales are distinct from the log P oct (partition coefficient in the octanol/H 2 O system) scale. With these objectives, the retention mechanisms on the IAM HPLC columns were analysed by linear solvation free‐energy relationships (LSERs). A set of 68 neutral model compounds with known solvatochromic parameters and log P oct values was investigated, allowing a regular and broad exploration of property space. The resulting solvatochromic equations clearly indicate that the three IAM stationary phases retain small neutral solutes by a balance of intermolecular forces closely resembling those underlying partitioning in octanol/H 2 O and retention on a reversed‐phase LC‐ABZ HPLC column. For all systems, the solute's size and hydrogen‐bond‐acceptor basicity are the two predominant factors, whereas dipolarity/polarisability and hydrogen‐bond‐donor acidity play only minor roles.