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A Useful Disulfide Linker for Single‐Bead Analysis of Peptide Libraries
Author(s) -
Lack Oliver,
Zbinden Hans,
Woggon WolfD.
Publication year - 2002
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/1522-2675(200202)85:2<495::aid-hlca495>3.0.co;2-e
Subject(s) - linker , chemistry , peptide , disulfide bond , combinatorial chemistry , hydrolysis , mass spectrometry , bead , tandem mass spectrometry , peptide synthesis , tandem , chromatography , organic chemistry , biochemistry , materials science , computer science , composite material , operating system
A disulfide‐linker for conventional peptide synthesis, attached to a PEGA‐resin, has been developed. Reductive hydrolysis cleaves the linker within minutes, liberating the synthesized peptide for rapid sequencing by tandem mass spectrometry. The method has been tested for ten peptides in a single‐bead fashion.