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Synthesis and Fluorescence Quenching Study of the Novel Cationic Probe Derived from Luminarosine
Author(s) -
Wenska Grażyna,
Skalski Bohdan,
TomskaForalewska Iwona,
Paszyc Stefan
Publication year - 2001
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/1522-2675(20011219)84:12<3726::aid-hlca3726>3.0.co;2-6
Subject(s) - chemistry , quenching (fluorescence) , cationic polymerization , fluorescence , nucleotide , aqueous solution , dna , halide , ion , binding constant , intermolecular force , photochemistry , molecule , binding site , inorganic chemistry , organic chemistry , biochemistry , physics , quantum mechanics , gene
A simple and efficient transformation of the zwitterionic luminarosine into a brightly fluorescent cationic analogue, namely 1‐amino‐9‐methoxy‐2,4,10‐triaza‐4b‐azoniaphenanthrene ( 3 ), is reported. The fluorescence quenching of 3 by common nucleotides, calf‐thymus (CT) DNA, and halide ions was investigated by means of spectrophotometric and spectrofluorometric methods. Intermolecular static and dynamic fluorescence‐quenching constants for quenching of 3 by nucleotides and halide ions were determined in aqueous solution. Evidence for formation of nonfluorescent ground‐state complexes of 3 with nucleotides and CT‐DNA is presented. Scatchard analysis of the CT‐DNA quenching data resulted in a binding constant of 2.8×10 4 M −1 and a number of binding sites per base pair of 0.049.