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Duplex‐Stabilization Properties of Oligodeoxynucleotides Containing N 2 ‐Substituted Guanine Derivatives
Author(s) -
Eritja Ramon,
Díaz Antonio R.,
SaisonBehmoaras Ester
Publication year - 2000
Publication title -
helvetica chimica acta
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.74
H-Index - 82
eISSN - 1522-2675
pISSN - 0018-019X
DOI - 10.1002/1522-2675(20000705)83:7<1417::aid-hlca1417>3.0.co;2-y
Subject(s) - chemistry , guanine , duplex (building) , protonation , stereochemistry , group (periodic table) , dna , combinatorial chemistry , nucleotide , organic chemistry , biochemistry , ion , gene
Oligodeoxynucleotides 3 – 13 carrying different guanine derivatives with substituents at the N 2 position have been prepared from a common precursor. Duplexes containing these modified bases are more stable than unmodified duplexes. The highest stability is found in guanine derivatives carrying at N 2 an ethyl and propyl group substituted with a group that is protonated under physiological conditions, which is compatible with a possible interaction of the protonatable group with the phosphates.