Premium
Asking Probing Questions: Can Fluorescent in situ Hybridization Identify and Localise Aquatic Hyphomycetes on Leaf Litter?
Author(s) -
McArthur Fiona A.,
Baerlocher Mark O.,
MacLean Natalie A.B.,
Hiltz Megan D.,
Bärlocher Felix
Publication year - 2001
Publication title -
international review of hydrobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.524
H-Index - 52
eISSN - 1522-2632
pISSN - 1434-2944
DOI - 10.1002/1522-2632(200107)86:4/5<429::aid-iroh429>3.0.co;2-9
Subject(s) - mycelium , hyphomycetes , conidium , biology , botany , microbiology and biotechnology
Based on partial sequences of 16S rRNA of 8 strains of the nectrioid group, a fluorescently labeled oligonucleotide was designed for in situ hybridization. It reacted with mycelia of 4 Heliscus lugdunensis strains, but not with mycelia of Cylindrocarpon destructans , Flagellospora curta , F. curvula , or Fusarium aquaeductuum . Much weaker signals were found with conidia. Staining with Rhodamine 123 showed that mitochondria in conidia were intact. When fungal mycelia were observed on alder or maple leaves, the specificity of the probe was maintained, but there was some interference due to autofluorescence of leaf parts and algal pigments.