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Activity Development of Selected Detoxication Enzymes during the Ontogenesis of the Zebrafish ( Danio rerio )
Author(s) -
Wiegand Claudia,
Pflugmacher Stephan,
Oberemm Axel,
Steinberg Christian
Publication year - 2000
Publication title -
international review of hydrobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.524
H-Index - 52
eISSN - 1522-2632
pISSN - 1434-2944
DOI - 10.1002/1522-2632(200008)85:4<413::aid-iroh413>3.0.co;2-3
Subject(s) - danio , detoxication , ontogeny , zebrafish , enzyme , glutathione , biology , glutathione peroxidase , hatching , biochemistry , chemistry , endocrinology , ecology , gene
Activities of two detoxication enzyme systems, microsomal and soluble glutathione S‐transferases (m‐/sGST, EC 2.5.1.18), and Se‐dependent glutathione peroxidase (GP‐X, EC 1.11.1.9) were monitored during zebrafish ( Danio rerio ) development. Starting with the 2–4 cell stage of ontogenesis successively up to adult fish, the presence of these detoxication enzymes could be demonstrated. Activities increased slowly during development and showed elevations corresponding to increased stress conditions. Plain increase occured after hatching, possibly caused by the change of the surrounding micromilieu. The juveniles showed highest activities, certainly provoked by new substances as from the food. Enzyme activities of adults revealed moderate levels probably due to a dilution of highly concentrated detoxication activities in the ready developed organs, especially the liver, in the whole body homogenate.