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Determination of free DNA in soils
Author(s) -
Niemeyer Jürgen,
Gessler Frank
Publication year - 2002
Publication title -
journal of plant nutrition and soil science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 87
eISSN - 1522-2624
pISSN - 1436-8730
DOI - 10.1002/1522-2624(200204)165:2<121::aid-jpln1111121>3.0.co;2-x
Subject(s) - chemistry , dna , soil water , centrifugation , microbiology and biotechnology , extraction (chemistry) , chromatography , biochemistry , biology , ecology
The concentration of free DNA in soils has to be determined in order to understand the fate and the transport of extracellular DNA. A protocol for the extraction and determination of free DNA was developed. The procedure uses separation steps, i.e. centrifugation and ultra‐filtration. The free dsDNA was stained with PicoGreen® and determined fluorimetrically. Samples from different soils, different soil horizons, soil waters and under different land use systems were analyzed. It was found that in nearly all samples free DNA was detectable. Free DNA concentrations of up to 1950 ng (g dried sample) —1 could be detected depending on depth, soil type and system of land use.