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Relative efficiency of fungal intra‐ and extracellular phosphatases and phytase
Author(s) -
Tarafdar Jagadish C.,
Yadav Ranjeet S.,
Niwas Ram
Publication year - 2002
Publication title -
journal of plant nutrition and soil science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.644
H-Index - 87
eISSN - 1522-2624
pISSN - 1436-8730
DOI - 10.1002/1522-2624(200202)165:1<17::aid-jpln17>3.0.co;2-c
Subject(s) - phytase , extracellular , phosphatase , acid phosphatase , chemistry , hydrolysis , alkaline phosphatase , biochemistry , intracellular , enzyme , microbiology and biotechnology , biology
A comparison of intra‐ and extracellular acid phosphatase, alkaline phosphatase and phytase activity in six fungi is reported. A strong linear relationship between intra versus extracellular fungal acid phosphatase (R 2 = 0.94), alkaline phosphatase (R 2 = 0.96), and phytase (R 2 = 0.97) is observed. Three‐fourth of acid phosphatase were generally present inside the fungal cells and only 25 % were released extracellularly after a three weeks period. Phytase shows the reverse trend where thirty nine times higher extracellular phytase activity was noticed than present inside the fungal cells. The extracellular enzymes are found 60 % more efficient in the hydrolysis of phytin than their intracellular counterpart but they are at par in the hydrolysis of glycerophosphate. The results clearly demonstrated that phytase types of phosphatases mostly occur outside the fungal cells whereas most of the acid phosphatase and alkaline phosphatase are located inside the cells.