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Postprocessing method to segregate and quantify the broad components underlying the phosphodiester spectral region of in vivo 31 P brain spectra
Author(s) -
Stanley Jeff A.,
Pettegrew Jay W.
Publication year - 2001
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/1522-2594(200103)45:3<390::aid-mrm1051>3.0.co;2-d
Subject(s) - phosphodiester bond , chemistry , anabolism , in vivo , spectroscopy , nuclear magnetic resonance , biological system , spectral line , molecule , biochemistry , physics , biology , genetics , gene , rna , organic chemistry , quantum mechanics , astronomy
In a typical, in vivo 31 P brain spectrum, the phosphomonoester (PME) and phosphodiester (PDE) spectral region not only contains signals from freely mobile PMEs and PDEs (which are anabolic and catabolic products of membrane phospholipids) but also signals of broader underlying lineshapes from less‐mobile molecules. In general, either the PME and PDE resonances are quantified as a combined value of freely mobile metabolites plus less‐mobile molecules or the broader underlying signal is reduced/eliminated prior to or post data collection. In this study, a postprocessing method that segregates and quantifies the individual contributions of the freely mobile metabolites and the less‐mobile molecules is introduced. To demonstrate the precision and accuracy of the method, simulated data and in vivo 31 P brain spectroscopy data of healthy individuals were quantified. The ability to segregate and quantify these various PME and PDE contributions provides additional spectral information and improves the accuracy of the interpretation of 31 P spectroscopy results. Magn Reson Med 45:390–396, 2001. © 2001 Wiley‐Liss, Inc.