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Determination of pH using water protons and chemical exchange dependent saturation transfer (CEST)
Author(s) -
Ward K.M.,
Balaban R.S.
Publication year - 2000
Publication title -
magnetic resonance in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.696
H-Index - 225
eISSN - 1522-2594
pISSN - 0740-3194
DOI - 10.1002/1522-2594(200011)44:5<799::aid-mrm18>3.0.co;2-s
Subject(s) - chemistry , proton , saturation (graph theory) , chemical shift , nuclear magnetic resonance , analytical chemistry (journal) , environmental chemistry , nuclear physics , physics , mathematics , combinatorics
Solution pH was measured using water proton NMR via chemical exchange dependent saturation transfer (CEST) with selected chemical exchange sites. Several useful pH‐sensitive proton chemical exchange agents were found: 5,6‐dihydrouracil, 5‐hydroxytryptophan, and a combination of 5‐hydroxytryptophan and 2‐imidazolidinethione. A ratiometric approach was developed that permitted pH determinations that were independent of water T 1 or exchange site concentration. Magn Reson Med 44:799–802, 2000. Published 2000 Wiley‐Liss, Inc.