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Eosin Y as an Internal Standard for a Plate Reader‐Based Quantitation of a Histone Deacetylase Substrate
Author(s) -
Heltweg Birgit,
Jung Manfred
Publication year - 2002
Publication title -
archiv der pharmazie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 61
eISSN - 1521-4184
pISSN - 0365-6233
DOI - 10.1002/1521-4184(200208)335:6<296::aid-ardp296>3.0.co;2-6
Subject(s) - histone deacetylase , substrate (aquarium) , chemistry , histone deacetylase inhibitor , histone , biochemistry , biology , gene , ecology
Ongoing interest in histone deacetylase (HDAC) inhibitors as potential anticancer drugs and mechanistic tools for the study of gene regulation is driving the improvement of assay techniques for the determination of HDAC activity. We previously reported the first non‐isotopic substrate for HDAC. A plate reader‐based determination of the substrate conversion utilized a boraindacene as an internal standard which is no longer commercially available. We report here that Eosin Y is a suitable replacement for that purpose, leading to a validated HDAC assay with increased throughput.