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Purified MHC class I molecules inhibit activated NK cells in a cell‐free system in vitro
Author(s) -
Kambayashi Taku,
Michaëlsson Jakob,
Fahlén Linda,
Chambers Benedict J.,
Sentman Charles L.,
Kärre Klas,
Ljunggren HansGustaf
Publication year - 2001
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/1521-4141(200103)31:3<869::aid-immu869>3.0.co;2-a
Subject(s) - lymphokine activated killer cell , biology , mhc class i , major histocompatibility complex , lymphokine , mhc restriction , microbiology and biotechnology , cd1 , receptor , natural killer cell , mhc class ii , cell , antigen presenting cell , cytotoxic t cell , in vitro , immunology , interleukin 21 , antigen , biochemistry
Abstract Natural killer cells have been shown to interact with MHC class I molecules via inhibitory receptors. However, it is not known whether the inhibition induced by MHC class I molecules requires other NK cell‐target cell interactions. Thus, we examined whether purified MHC class I molecules alone were able to inhibit NK cell function. Purified H‐2K b and H‐2D b molecules inhibited the release of IFN‐γ from spleen (H‐2 b )‐derived lymphokine‐activated killer (LAK) cell cultures stimulated by anti‐NK1.1 antibody in a concentration‐dependent manner. LAK cells generated from newborn mice that express low levels of MHC class I binding Ly49 inhibitory receptors were significantly less sensitive to inhibition by H‐2K b compared to LAK cells from adult mice. Furthermore, LAK cells generated from spleen cells of Ly49C‐transgenic mice were significantly more sensitive to inhibition by H‐2K b compared to non‐transgenic littermates. Taken together, the data indicate that MHC class I induced inhibition of NK cell mediated effector functions, as assessed by IFN‐γ release after NK1.1 triggering, does not require additional cell surface molecules other than MHC class I.