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Regulation of cell survival during B lymphopoiesis: increased pre‐B cell apoptosis in CD24‐transgenic mouse bone marrow
Author(s) -
Lu Liwei,
Chappel M. Suzanne,
Humphries R. Keith,
Osmond Dennis G.
Publication year - 2000
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/1521-4141(200009)30:9<2686::aid-immu2686>3.0.co;2-f
Subject(s) - lymphopoiesis , biology , apoptosis , bone marrow , b cell , flow cytometry , cd24 , b 1 cell , microbiology and biotechnology , immunology , haematopoiesis , t cell , stem cell , antibody , antigen presenting cell , immune system , biochemistry , cancer stem cell
Abstract CD24 (heat‐stable antigen) is expressed in a developmentally regulated fashion by B cell precursors in mouse bone marrow (BM), but its role in B lymphopoiesis remains obscure. A slight overexpression of CD24 in transgenic (Tg) mice leads to depletion of B lymphoid cells in BM. The present study examines whether CD24 is involved in apoptotic selection of B lineage cells under normal microenvironmental conditions in vivo . Double immunofluorescence labeling and flow cytometry have been used to quantitate the apoptotic rates of phenotypically defined B cell populations in BM of CD24‐Tg mice. Apoptosis of pre‐B cells expressing cytoplasmic μ heavy chains of IgM but lacking surface (s)IgM was increased both ex vivo and in short‐term culture, while the number of pre‐B cells was halved compared to BM of normal mice. In contrast, B220 + μ – pro‐B cells and sIgM + B lymphocytes showed no significant change in either apoptosis or number. The findings provide evidence that CD24 can play a role in vivo in modulating pre‐B cell apoptosis, a quality control checkpoint in B cell development.