Premium
Simultaneous Determination of Tramadol and Lidocaine in Urine by End‐column Capillary Electrophoresis with Electrochemiluminescence Detection
Author(s) -
Cao Weidong,
Liu Jifeng,
Qiu Haibo,
Yang Xiurong,
Wang Erkang
Publication year - 2002
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/1521-4109(200211)14:22<1571::aid-elan1571>3.0.co;2-x
Subject(s) - tramadol , lidocaine , chromatography , chemistry , urine , detection limit , capillary electrophoresis , analgesic , anesthesia , medicine , biochemistry
Tramadol and lidocaine, used as analgesic and local anesthetic in surgery, are partly excreted by kidney. For the first time, we developed a simple and sensitive method, based on capillary electrophoresis with electrochemiluminescence (ECL) detection by end column mode without joint to monitor tramadol and lidocaine in urine. To eliminate the influence of ionic strength of urine sample, analytes were extracted by ether. Tripropylamine (TPA) was used as internal standard. The recoveries of tramadol and lidocaine were between 94% and 97% at different levels. The method exhibited the linear range for the tramadol and lidocaine from 1.0×10 −7 to 1.0×10 −4 mol/L with correlation efficient of 0.998. The relative standard deviation (RSD) was 2.9% and 2.7% ( n =8) for tramadol and lidocaine, respectively. The limit of detection (LOD) was 6.0×10 −8 mol/L and 4.5×10 −8 mol/L (S/N=3) for tramadol and lidocaine, respectively. The application for detecting tramadol and lidocaine in urine of patients showed that the method was valuable in clinical and biochemical laboratories for detecting tramadol, lidocaine and other tertiary amine pharmaceuticals for various purpose, such as metabolism investigation.