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Detection of Chemically‐Induced Damage in Layered DNA Films with Co(bpy) 3 3+ by Square‐Wave Voltammetry
Author(s) -
Yang Jing,
Zhang Zhe,
Rusling James F.
Publication year - 2002
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/1521-4109(200211)14:21<1494::aid-elan1494>3.0.co;2-i
Subject(s) - pyrolytic carbon , styrene oxide , dna damage , chemistry , dna , voltammetry , metabolite , adduct , cyclic voltammetry , electrode , inorganic chemistry , nuclear chemistry , styrene , organic chemistry , biochemistry , electrochemistry , polymer , pyrolysis , copolymer
Damage of double stranded (ds) DNA by metabolites of lipophilic pollutants and drugs constitutes a major toxicity pathway. We evaluated films of DNA and poly(dimethyldiallylammonium chloride) (PDDA) grown layer‐by‐layer on pyrolytic graphite electrodes for the detection of DNA damage by the model metabolite styrene oxide, which forms chemical adducts with DNA bases. The method features electroactive probe Co(bpy) 3 3+ , which binds to films with DNA as the outer layer [(PDDA/ds‐DNA) 2 ] in greater amounts when the ds‐DNA is intact than when it is damaged. Square‐wave voltammetry peaks of Co(bpy) 3 3+ decreased with incubation time of (PDDA/DNA) 2 electrodes in styrene oxide solutions, while no changes in the peaks were found for control incubations with non‐reactive toluene. Comparison with capillary electrophoretic analyses suggested that this voltammetric method can detect roughly 0.05% damage. The presence of hemoprotein in the films did not interfere with the analysis. The method appears promising for the rapid detection of relative rates of DNA damage by human liver metabolites.