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Comparison of Voltammetry Assisted by Multivariate Analysis with EXAFS as Applied to the Study of Cd‐ and Zn‐Binding of Metallothionein Related Peptides
Author(s) -
DíazCruz M. S.,
DíazCruz J. M.,
Esteban M.
Publication year - 2002
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/1521-4109(200207)14:13<899::aid-elan899>3.0.co;2-8
Subject(s) - extended x ray absorption fine structure , chemistry , metallothionein , voltammetry , glutathione , cyclic voltammetry , metal , inorganic chemistry , cadmium , absorption spectroscopy , electrochemistry , biochemistry , organic chemistry , enzyme , electrode , quantum mechanics , physics
Zn‐ and Cd‐complexes of glutathione (GSH) and the metallothionein related peptide Lys‐Cys‐Thr‐Cys‐Cys‐Ala (FT) were investigated by EXAFS and by voltammetry, assisted by multivariate curve resolution (MCR). EXAFS data from the Zn‐FT system seem to show the formation in low extent (under 50%) of a metal cluster with a coordination number of four. In the cases of Cd‐FT, Zn‐GSH and Cd‐GSH no sound conclusions can be deduced from EXAFS. These results are in contrast with those found by voltammetric techniques. The use of MCR allows to calculate the voltammograms corresponding to each single process and its corresponding concentration profile. Then models of complexation are proposed.