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Electron Transfer Reaction of Cytochrome c at the Electrode Surface Analyzed with Noncontact Optical Waveguide Spectroscopy
Author(s) -
Fukuda Kyoko,
Ohno Hiroyuki
Publication year - 2002
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/1521-4109(200205)14:9<605::aid-elan605>3.0.co;2-w
Subject(s) - redox , absorbance , electron transfer , electrode , chemistry , photochemistry , cytochrome c , adsorption , spectroscopy , analytical chemistry (journal) , absorption spectroscopy , hemeprotein , electrochemistry , heme , inorganic chemistry , optics , organic chemistry , chromatography , biochemistry , physics , quantum mechanics , mitochondrion , enzyme
We proposed a unique application of optical waveguide (OWG) spectroscopy under noncontact condition to analyze the electrochemical redox reaction of molecules on the electrode surface. The spectral change of cytochrome c (cyt.c), induced by the potential switching, was clearly detected. In addition, the absorbance change at the Soret band and Q‐band was recorded dynamically with this apparatus. The absorbance change was revealed to simply reflect the electron transfer reaction of cyt.c at the electrode surface. The magnitude of the intensity change was gradually diminished suggesting some cyt.cs were adsorbed on the electrode surface and suppressed further redox reaction of the dissolved cyt.cs. The noncontact measurement enables to take the absorption spectra of only the adsorbed cyt.c on the electrode. The adsorbed cyt.c was revealed to be denatured containing ferric ion in the heme and the redox activity was completely lost.