Premium
Determination of Histamine by Capillary Zone Electrophoresis with End‐Column Amperometric Detection at a Carbon Fiber Microdisk Array Electrode
Author(s) -
Weng Qianfeng,
Xia Fangquan,
Jin Wenrui
Publication year - 2001
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/1521-4109(200111)13:17<1459::aid-elan1459>3.0.co;2-y
Subject(s) - capillary electrophoresis , detection limit , amperometry , chromatography , electrophoresis , electrode , analytical chemistry (journal) , chemistry , buffer (optical fiber) , capillary action , working electrode , materials science , electrochemistry , telecommunications , composite material , computer science
Capillary zone electrophoresis (CZE) was employed for the analysis of histamine using an end‐column amperometric detector with a carbon fiber microdisk array electrode. The optimum conditions of separation and detection are: 40 mmol/L NaH 2 PO 4 –Na 2 HPO 4 (pH 7.0) for the buffer solution, 12.5 kV for the separation voltage, 5 kV and 10 s for the injection voltage and the injection time, and 1.30 V (vs. SCE) for the detection potential. The limit of detection is 3.3×10 −6 mol/L or 13 fmol (signal to noise, S / N =3). The relative standard derivation was 0.45% for the migration time and 3.2% for the electrophoretic peak current. The method can be applied to determining histamine in rat peritoneal mast cells. The recovery of the method is between 92 and 99%.