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An Electrogenerated Chemiluminescence Imaging Fiber Electrode Chemical Sensor for NADH
Author(s) -
Jin Eunsook S.,
Norris Bradley J.,
Pantano Paul
Publication year - 2001
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/1521-4109(200110)13:15<1287::aid-elan1287>3.0.co;2-i
Subject(s) - electrode , triethylamine , chemiluminescence , materials science , nafion , analytical chemistry (journal) , layer (electronics) , chemistry , electrochemistry , nanotechnology , chromatography , organic chemistry
An electrogenerated chemiluminescence (ECL)‐based imaging fiber electrode chemical sensor (IFECS) for the detection of NADH is presented. In brief, an imaging fiber's distal tip was metalized with gold to serve as an electrode (i.e., an IFE). The 350‐μm diameter IFE was coated with a thin, planar layer of Nafion doped with Ru(bpy) 3 2+ to create an IFECS. An electrical contact was made to the gold layer such that Ru(bpy) 3 2+ could be oxidized to Ru(bpy) 3 3+ and electrochemically regenerated following the application of appropriate potentials. Following the diffusion of triethylamine or NADH into the IFE sensing layer and reaction with Ru(bpy) 3 3+ , the ECL was captured using a charge coupled device‐based imaging system. The triethylamine calibration plot was linear between 0.0 and 50 mM. IFECSs represent the first demonstration whereby ECL imaging is performed through the actual sensor itself.