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Use of Thermally Simulated Aging to Evaluate the Contribution of Constituents to the Activity of Mediated Carbon Paste Glucose Biosensors to Improve Their Shelf‐Life
Author(s) -
Szuwarski Nadine,
Gueguen Sophie,
Boujtita Mohamed,
El Murr Nabil
Publication year - 2001
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/1521-4109(200110)13:15<1237::aid-elan1237>3.0.co;2-s
Subject(s) - glucose oxidase , ferrocene , electrode , shelf life , biosensor , chemistry , carbon fibers , chemical engineering , matrix (chemical analysis) , hydroxymethyl , carbon paste electrode , materials science , electrochemistry , organic chemistry , chromatography , cyclic voltammetry , biochemistry , composite material , food science , composite number , engineering
The aging effect on the activity of the carbon paste electrode modified with glucose oxidase and ferrocene as redox mediator was studied. This study had been achieved using an artificial aging model in order to simulate the shelf‐life of the enzyme electrode in a short time period. The activity of carbon pastes modified with glucose oxidase (GOD) and ferrocene (FcH) or hydroxymethyl ferrocene (FcH 2 OH) was evaluated and compared at different storage times at 55 °C in dried conditions. Two different binders containing FcH or FcCH 2 OH were also examined in order to evaluate the effect of the pasting oil on the shelf‐life of the electrode. The results showed that the proposed model give in a very short time an accurate prediction for the determination of the storage stability and the activity of stocked electrodes. Contrarily to what could be expected at first sight, the study of the aging effect on the different components of the modified carbon paste electrode showed that the decrease of the surface activity of the electrode was not related to a simple enzymatic deactivation. This indicates that the enzyme shows a high stability in the carbon paste matrix.