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Amperometric Biosensor for Hydrogen Peroxide Based on Sol‐Gel/Hydrogel Composite Thin Film
Author(s) -
Chen Xu,
Wang Bingquan,
Dong Shaojun
Publication year - 2001
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/1521-4109(200110)13:14<1149::aid-elan1149>3.0.co;2-l
Subject(s) - hydrogen peroxide , amperometry , horseradish peroxidase , biosensor , sol gel , detection limit , tetrathiafulvalene , vinyl alcohol , chemistry , materials science , nuclear chemistry , chemical engineering , chromatography , electrochemistry , electrode , organic chemistry , nanotechnology , polymer , molecule , engineering , enzyme
A reagentless amperometric hydrogen peroxide biosensor was developed. Horseradish peroxidase (HRP) was immobilized in a novel sol‐gel organic‐inorganic hybrid matrix that is composed of silica sol and a grafting copolymer of poly(vinyl alcohol) with 4‐vinylpyridine (PVA‐g‐PVP). Tetrathiafulvalene (TTF) was employed as a mediator and could lower the operating potential to −50 mV (versus Ag/AgCl). The sensor achieved 95 % of the steady‐state current in 15 s. Linear calibration for hydrogen peroxide was up to 1.3 mM with the detection limit of 2.5×10 −7 M. The enzyme electrode retained about 94 % of its initial activity after 30 days of storage in a dry state at 4 °C.