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Biotinylated Polypyrrole Modified Quartz Crystal Microbalance for the Fast and Reagentless Determination of Avidin Concentration
Author(s) -
Cosnier Serge,
Perrot Hubert,
Wessel Rudolf
Publication year - 2001
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/1521-4109(200107)13:11<971::aid-elan971>3.0.co;2-d
Subject(s) - quartz crystal microbalance , avidin , biotinylation , polypyrrole , chemistry , electrode , materials science , polymerization , analytical chemistry (journal) , chromatography , polymer , organic chemistry , biochemistry , adsorption
A quartz crystal microbalance (QCM) bioaffinity sensor for avidin, based on electropolymerized biotinylated polypyrrole film, was developed. The functionalization of one of the Au quartz electrode has been achieved by controlled potential oxidation of a biotinyl pyrrole monomer in CH 3 CN. The modified quartz resonator was placed in a QCM flow cell. In the presence of avidin solutions, gravimetric measurements indicated the reproducible formation of a compact avidin layer via the specific avidin‐polymerized biotin interaction. The polymerized biotin retained identical bioaffinity properties for avidin over a period of one month. The initial slope of the frequency change as a function of time was recorded and related to avidin concentrations. Avidin concentration in the range 1.5×10 −9 –3.7×10 −5 M can be measured by this method.