Premium
Constant‐Current‐Mode LAPS (CLAPS) for the Detectionof Penicillin
Author(s) -
Yoshinobu Tatsuo,
Ecken Holger,
Poghossian Arshak,
Simonis Anette,
Iwasaki Hiroshi,
Lüth Hans,
Schöning Michael J.
Publication year - 2001
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/1521-4109(200105)13:8/9<733::aid-elan733>3.0.co;2-n
Subject(s) - constant current , current (fluid) , chemistry , constant (computer programming) , potentiometric titration , time constant , analytical chemistry (journal) , penicillin , electroanalytical method , materials science , chromatography , electrode , computer science , electrical engineering , physics , thermodynamics , engineering , biochemistry , programming language , antibiotics
A software feedback control system for the constant‐current‐mode operation of the light‐addressable potentiometric sensor (LAPS) was developed. The constant‐current‐mode LAPS (CLAPS) is suitable for online monitoring and recording of changes in the pH value or the ion concentration. An enzyme LAPS was fabricated by adsorptive immobilization of penicillinase on the pH‐sensitive layer of Ta 2 O 5 . This sensor was operated in the constant‐current‐mode and the detection limit for penicillin G was found to be at least as low as 100 µM.