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A General Method for the Electrochemical Evaluation of the Bimolecular Rate Constant in Enzyme Catalyzed Reaction Kinetics
Author(s) -
Antiochia Riccarda,
Lavagnini Irma,
Magno Franco
Publication year - 2001
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/1521-4109(200105)13:7<601::aid-elan601>3.0.co;2-h
Subject(s) - reaction rate constant , chemistry , diffusion , kinetics , catalysis , substrate (aquarium) , electrochemistry , reaction rate , enzyme kinetics , limiting current , limiting , constant (computer programming) , kinetic energy , thermodynamics , analytical chemistry (journal) , chromatography , organic chemistry , electrode , active site , physics , quantum mechanics , computer science , programming language , mechanical engineering , oceanography , engineering , geology
A general working curve in terms of the ratio of the kinetic limiting current of bioelectrocatalysis to the diffusion‐controlled current recorded in the absence of the enzyme reaction versus the k [E] ( k =bimolecular rate constant, [E]=enzyme concentration) group at high concentration of the substrate and at very low mediator concentration was constructed. The curve allows one to evaluate the bimolecular catalytic constant for one‐ or two‐electron mediator from voltammetric data recorded at a fixed scan rate.