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Electrochemical Monitoring of Plant Stress Responses
Author(s) -
Kinpara Takeshi,
Murakami Yuji,
Yokoyama Kenji,
Tamiya Eiichi
Publication year - 2001
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/1521-4109(200104)13:6<451::aid-elan451>3.0.co;2-9
Subject(s) - hydrogen peroxide , ferricyanide , electrochemistry , chemistry , salicylic acid , amperometry , nicotiana tabacum , nuclear chemistry , inorganic chemistry , electrode , biochemistry , gene
Intact cultured cells of Nicotiana tabacum BY2 cells exhibited the electrochemical reduction of 1,4‐benzoquinone (BQ), 2,6‐dichloroindophenol (DCIP), hexacyanoferrate (ferricyanide). This was detected by amperometry with fixed potential (400 mV vs. Ag/AgCl) using a film‐coated ø 1.6 mm platinum disk electrode as a working electrode. The electrochemical activities of BQ, DCIP, and ferricyanide mixed with BY2 cell were changed after the addition of various stress compounds such as elicitors, plant hormones, metabolic and electron transport inhibitors. Different stress compounds gave different electrochemical patterns probably due to the different positions of electron acceptors affected by these bio‐materials. In addition, we observed the generation of hydrogen peroxide in BY2 cells treated with various stress compounds. The pattern of chemiluminescence is useful for the determination of the intensity of plant stress responses. High accumulation of hydrogen peroxide was observed in the BY2 cells treated with elicitors (salicylic acid and ethylene glycol chitin) and plant hormone (2,4‐D). A relationship between electrochemical patterns and stress compounds were also discussed.