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Effect of DNA on the Peroxidase Based Biosensor for Phenol Determination in Waste Waters
Author(s) -
Rosatto Simone S.,
de Oliveira Neto Graciliano,
Kubota Lauro T.
Publication year - 2001
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/1521-4109(200104)13:6<445::aid-elan445>3.0.co;2-y
Subject(s) - biosensor , polyethylenimine , phenol , chemistry , chromatography , bovine serum albumin , peroxidase , detection limit , nuclear chemistry , organic chemistry , biochemistry , enzyme , transfection , gene
The influence of different additives, like bovine serum albumin (BSA), polyethylenimine (PEI) and DNA, on the sensitivity and operational stability of a phenol biosensor based on peroxidase immobilized on silica‐titanium and mixed in carbon was studied. The best results were found with electrodes containing DNA in the paste, although with BSA better results were obtained, compared to the electrode without additives. The response of biosensor incorporating DNA decayed ca. 50 % after 3 to 4 days of use, but it then remained constant for 25 days of daily use, when stored in a phosphate buffer solution under refrigeration between experiments. Original performance was recovered by simply substituting the used paste for a new portion of stock paste. The high sensitivity for phenol due to the immobilization of peroxidase on silica‐titanium and the increase of stability of the biosensor provided by DNA addition were essential for the successful determination of phenol in real samples, with an average of 104.8 % recovery.