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Evaluation of Heat Resistance of Microorganisms by an Amperometric Measurement of the Microbial Substrate‐Oxidizing Activity Using a Whole‐CellModified Electrode
Author(s) -
Kondo Tetsuya,
Ando Toshiyuki,
Ikeda Tokuji
Publication year - 2001
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/1521-4109(200104)13:5<392::aid-elan392>3.0.co;2-v
Subject(s) - oxidizing agent , electrode , incubation , lactobacillus plantarum , substrate (aquarium) , microorganism , chemistry , escherichia coli , amperometry , incubation period , microbiology and biotechnology , bacteria , chromatography , biochemistry , biology , electrochemistry , lactic acid , organic chemistry , ecology , gene , genetics
A mediated bioelectrocatalysis using a bacterial cell‐modified electrode was applied to the evaluation of heat resistance of Escherichia coli and Lactobacillus plantarum . The first derivative of the time course of the bioelectrocatalysis current for the bacterial D ‐glucose oxidation had a maximum d I /d t max at about 10 to 60 s after the addition of D ‐glucose to the solution. The d I /d t max value decreased in different manners depending on the conditions of heat treatment of the bacterial cells prior to their immobilization on electrode. The decreasing tendency in the d I /d t max value was similar to that in the colony formation unit measured by a conventional colony counting method. The d I /d t max method, which takes only 2 h, is much more time saving compared with the conventional method taking 24 h incubation for E. coli and 72 h incubation for L. plantarum . In some cases, however, there was a difference in the magnitude of relative decrease between the two methods, which could be attributed to the appearance of injured cells that were active in the d I /d t max measurement but not in the colony formation.