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Glucose‐Sensing Electrode Based on Glucose Oxidase‐Attached Polyion Complex Membrane Containing Peroxidaseand Ferrocene
Author(s) -
Yabuki Soichi,
Mizutani Fumio,
Hirata Yoshiki
Publication year - 2001
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/1521-4109(200104)13:5<380::aid-elan380>3.0.co;2-6
Subject(s) - glucose oxidase , ferrocene , glutaraldehyde , electrode , chemistry , membrane , biosensor , immobilized enzyme , nuclear chemistry , inorganic chemistry , chromatography , electrochemistry , organic chemistry , enzyme , biochemistry
A glucose oxidase (GOD)‐attached polyion complex membrane containing peroxidase (POD) and ferrocene was prepared by two steps on a glassy carbon electrode. First, a polyion complex membrane containing POD and ferrocene was prepared: a poly‐ L ‐lysine solution, a POD solution, a ferrocene dispersion and a poly(styrenesulfonate) solution were successively dropped on an electrode, and the electrode was allowed to dry. Then GOD was attached by the use of glutaraldehyde: GOD and glutaraldehyde solutions were dropped on the membrane, and the electrode was dried. Current response to glucose for the enzyme electrode was measured at a potential of −0.2 V (vs. Ag/AgCl). The 100 % response time was ca. 20 s. The current was proportional to the glucose concentration up to 0.1 mM. The lower detection limit was 1 µM (signal‐to‐noise ratio, S / N =5). The enzyme electrode was used for the glucose measurements in beverages. The results obtained agreed well with those obtained by Boehringer F‐kit method. The electrode would be useful as a glucose biosensor for beverages.