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Amperometric Glucose Biosensor Based on Immobilization of Glucose Oxidase in Chitosan Matrix Cross‐Linked with Glutaraldehyde
Author(s) -
Miao Y.,
Chia L. S.,
Goh N. K.,
Tan S. N.
Publication year - 2001
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/1521-4109(200103)13:4<347::aid-elan347>3.0.co;2-z
Subject(s) - biosensor , glucose oxidase , glutaraldehyde , chitosan , amperometry , chemistry , chromatography , matrix (chemical analysis) , electrode , ferrocene , electrochemistry , organic chemistry , biochemistry
A glucose biosensor was fabricated using chitosan film for the immobilization of glucose oxidase (GOX) and mediator in the following “sandwich” configuration:chitosan‐ferrocene:GOX:chitosan using a carbon paste electrode as the basic electrode. To prevent loss of immobilized GOX under optimized conditions, the biosensor surface was cross‐linked with glutaraldehyde (GA). Despite the multilayer configuration, the biosensor exhibited a relatively fast response (less than 10 s) and a linear response with glucose in the range of 8×10 −4 to 1.7×10 −2 M. The biosensor retained about 65 % of its original activity after 30 days of storage in a phosphate buffer at 4 °C. Applicability of the biosensor was demonstrated in real samples and the results obtained by this biosensor corroborate well with a classical UV spectrophotometric technique.