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Mitochondria‐Based Amperometric Biosensor for the Determination of L ‐Glutamic Acid
Author(s) -
Girousi S. T.,
Pantazaki A. A.,
Voulgaropoulos A. N.
Publication year - 2001
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/1521-4109(200103)13:3<243::aid-elan243>3.0.co;2-j
Subject(s) - amperometry , biosensor , glutamic acid , chemistry , detection limit , electrochemistry , linear range , chromatography , electrode , nuclear chemistry , analytical chemistry (journal) , biochemistry , amino acid
A mitochondria based amperometric biosensor was developed for the determination of L ‐glutamic acid. The biosensor was characterized using hexacyanoferrate(III) as the electrochemical mediator. The electrochemical behavior of the redox couple hexacyanoferrate(II)/(III) was improved by incorporating octadecylamine into the enzyme modified carbon paste. L ‐Glutamic acid was detected at +0.35 V. Linearity was observed in the range 0.4–10 mM of L ‐glutamic acid (sensitivity 0.189 µA/mM, correlation coefficient 0.997) and in the range 10–100 mM (sensitivity 0.021 µA/mM, correlation coefficient 0.958). The detection limit was 0.1 mM. The electrode maintains 60 % of its activity after 10 days of use. The bioelectrode was used for the determination of L ‐glutamate in chicken cube samples.