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Sonoelectroanalytical Determination of Heavy Metals in Fish Gill Mucous
Author(s) -
L. Hardcastle Joanna,
G. Compton Richard
Publication year - 2001
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/1521-4109(200102)13:2<89::aid-elan89>3.0.co;2-z
Subject(s) - anodic stripping voltammetry , chemistry , copper , mercury (programming language) , heavy metals , materials science , electrode , electrochemistry , environmental chemistry , metallurgy , computer science , programming language
Fish gill mucous is used as a nondestructive biomarker for the detection of heavy metals by sono‐square‐wave anodic stripping voltammetry (sono‐SWASV). The mucous sample was obtained by gently rinsing the rainbow trout ( Salmo gairdneri ) with a solution of borate buffer at pH 7. A glassy carbon electrode plated in situ with mercury was used in a face on arrangement with the ultrasonic horn to first demonstrate that voltammetric measurements can accurately be made in borate buffer. The optimum deposition potential and ultrasound power for copper from a dilute mucous solution was then determined and used to give a quantitative assessment of copper content using the microaddition technique. The results had a % RSD of less than 2 % and compared favorably with an independent blind analysis. The potential of the technique for detection of other heavy metals for example lead, was also demonstrated. We conclude that sono‐SWASV of gill mucous may present a novel alternative to the destructive conventional techniques due to its accuracy, rapidity and facile methodology.