Premium
Immunoassay of Staphylococcal Enterotoxin C1 by FTIR Spectroscopy and Electrochemical Gold Electrode
Author(s) -
Dong Saying,
Luo Guoan,
Feng Jun,
Li Qingwen,
Gao Hong
Publication year - 2001
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/1521-4109(200101)13:1<30::aid-elan30>3.0.co;2-t
Subject(s) - immunoassay , dielectric spectroscopy , electrode , fourier transform infrared spectroscopy , chemistry , enterotoxin , colloidal gold , substrate (aquarium) , adsorption , electrochemistry , analytical chemistry (journal) , materials science , chromatography , antibody , nanoparticle , nanotechnology , chemical engineering , biochemistry , escherichia coli , immunology , oceanography , engineering , biology , organic chemistry , gene , geology
A self‐assembled gold surface acting as a substrate, a kind of antibody immunosensor is described. This sensor is composed of a three‐electrode system and a layer of antibody of staphylococcal enterotoxin C1(SEC1). The antigen of SEC1 is detected by an electrochemical method at a constant antibody concentration. The redox probe is used to determine the extent of the biolayer insulation. AC impedance spectroscopy is fast and simple and it can be applied as an electrochemical sensing device for the antigen. Fourier transform IR spectroscopy is used to confirm the adsorption of the SEC1 antibody on the gold electrode and the association of SEC1 antigen and antibody.