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Mercury Film Electrode as a Sensor for the Detection of DNA Damage
Author(s) -
Kubičárová Tatiana,
Fojta Miroslav,
Vidic Jasmina,
Havran Luděk,
Paleček Emil
Publication year - 2000
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/1521-4109(200011)12:17<1422::aid-elan1422>3.0.co;2-c
Subject(s) - mercury (programming language) , dna , biosensor , restriction enzyme , electrode , cleavage (geology) , deoxyribonuclease , plasmid , cyclic voltammetry , voltammetry , chemistry , endonuclease , materials science , biochemistry , electrochemistry , computer science , fracture (geology) , composite material , programming language
Cleavage of supercoiled (sc) plasmid DNA by restriction endonucleases and by deoxyribonuclease I was detected by means of AC voltammetry at mercury film on glassy carbon electrode (MFE). Using this technique, it was possible to detect both single‐ and double‐strand breaks in DNA. MFE modified with scDNA was used as a biosensor for the detection of DNA‐cleaving species.