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Distinction Between Native and Denatured DNA by Means of Compression
Author(s) -
Novotný Ladislav,
Fojta Miroslav,
Heyrovský Michael
Publication year - 2000
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/1521-4109(200010)12:15<1233::aid-elan1233>3.0.co;2-j
Subject(s) - hanging mercury drop electrode , electrode , dna , cyclic voltammetry , compression (physics) , drop (telecommunication) , mercury (programming language) , voltammetry , analytical chemistry (journal) , ion , chemistry , adsorption , electrochemistry , materials science , inorganic chemistry , chromatography , composite material , organic chemistry , biochemistry , telecommunications , computer science , programming language
Native and denatured DNA show different behavior on a compression mercury drop electrode in voltammetric and chronoamperometric measurements. The effect of both forms of adsorbed DNA on cyclic voltammetry of Cd 2+ ions were followed in course of the growth, compression and repeated growth of the mercury electrode. The measurements have indicated different compression ‐expansion properties of the two DNA forms and possibilities of their voltammetric distinction in course of tens of seconds up to a few minutes. Different behavior was also observed on compression i – t curves after DNA accumulation on slowly growing or stationary mercury drop electrode. Most marked differences between i – t curves were observed in the potential region around –0.6 V (vs. SCE) where DNA is strongly adsorbed.