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Amperometric Biosensor for Phenols and Catechols Based on Iridium‐Polyphenol Oxidase‐Modified Carbon Paste
Author(s) -
Rubianes M. D.,
Rivas G. A.
Publication year - 2000
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/1521-4109(200010)12:14<1159::aid-elan1159>3.0.co;2-n
Subject(s) - phenols , amperometry , chemistry , iridium , polyphenol oxidase , catechol , biosensor , carbon paste electrode , carbon fibers , polyphenol , nuclear chemistry , organic chemistry , combinatorial chemistry , chromatography , electrode , electrochemistry , cyclic voltammetry , enzyme , materials science , catalysis , biochemistry , antioxidant , peroxidase , composite material , composite number
An amperometric bioelectrode for the quantification of phenols and catechols based on the incorporation of iridium microparticles and polyphenol oxidase (PPO) within carbon paste matrices, is described. Iridium‐doped carbon paste electrode displays excellent electrocatalytic activity towards the reduction of the enzymatically formed quinone species. Thus, the quantification of the micromolar/submicromolar levels of phenols and catechols can be performed at potentials lower than in the case of bare CPE with much better sensitivity (around 4‐fold higher). The analytical performance as well as the kinetic parameters for different substrates of clinical, pharmacological and environmental interest, are discussed.