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Portable Immunosensor for the Fast Amperometric Detection of Anti‐Hantavirus Antibodies
Author(s) -
Vetcha Srinivas,
AbdelHamid Ihab,
Atanasov Plamen,
Ivnitski Dmitri,
Wilkins Ebtisam,
Hjelle Brian
Publication year - 2000
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/1521-4109(200009)12:13<1034::aid-elan1034>3.0.co;2-l
Subject(s) - amperometry , immunoassay , recombinant dna , virology , hantavirus , antibody , antigen , chromatography , specific antibody , virus , chemistry , medicine , immunology , electrochemistry , biochemistry , electrode , gene
Abstract An amperometric immunosensor based on a recombinant nucleocapsid antigen has been developed for fast determination of antibodies against Sin Nombre (SN) virus in human blood serum. This approach combines the advantages of utilization of highly dispersed immunosorbent, with highly sensitive electrochemical detection of enzyme‐marker, and flow‐injection scheme of immunoassay. A main benefit of recombinant nucleocapsid antigen is that antigen preparation and standardization is easy. The complete immunoassay can be performed in 24 minutes. The short overall assay time could be a major advantage in the field of diagnostic screening of infectious diseases in mobile laboratories and small hospitals. The immunosensor has been successfully used in the determination of anti‐Hantavirus IgG in human blood plasma and is easily adaptable for the detection of other types of viral infections.