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Reactivities of Organic Phase Biosensors 3: Electrochemical Study of Cytochrome P450 cam Immobilized in a Methyltriethoxysilane Sol‐Gel
Author(s) -
Iwuoha Emmanuel I.,
Kane Stephen,
Ania Conchita Ovin,
Smyth Malcolm R.,
Ortiz de Montellano Paul R.,
Fuhr Uwe
Publication year - 2000
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/1521-4109(200008)12:12<980::aid-elan980>3.0.co;2-f
Subject(s) - cyclic voltammetry , chemistry , acetonitrile , biosensor , amperometry , electrochemistry , bromide , aqueous solution , phase (matter) , glutaraldehyde , chromatography , nuclear chemistry , electrode , inorganic chemistry , organic chemistry , biochemistry
A composite amperometric biosensor was prepared by encapsulating a cytochrome P450 cam /didodecyldimethylammonium bromide (DDAB) liquid crystal system in a methyltriethoxysilane (MTEOS) sol‐gel (SG). The composite sensing layer was cross‐linked unto a 0.071 cm 2 glassy carbon electrode (GCE) using glutaraldehyde. The electrochemical characteristics of the GCE‐[P450 cam /DDAB/SG] biosensor were studied by multiscan cyclic voltammetry in aqueous phase (0.05 phosphate buffer pH 7.3) and organic (CH 3 CN : H 2 0; 90 % : 10 %) phase anaerobic media. Analysis of the cyclic voltammetric results show that the sensor exhibited faster reversible electrochemistry in the organic phase than in the aqueous phase. The cyclic voltammetric peak separation, Δ E p , values estimated for the buffer and acetonitrile media were 62 mV and 104 mV, respectively. The sol‐gel‐based sensor had a life‐time of 4 weeks when stored at 4 °C, and was stable up to 45 °C, which is also the temperature of optimal sensor activity.