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Electrochemical Oxidation of 8‐Oxoguanine
Author(s) -
Brett A. M. Oliveira,
Piedade J. A. P.,
Serrano S. H. P.
Publication year - 2000
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/1521-4109(200008)12:12<969::aid-elan969>3.0.co;2-o
Subject(s) - guanine , electrochemistry , dna oxidation , dna , chemistry , detection limit , glassy carbon , electrode , dna damage , combinatorial chemistry , biochemistry , cyclic voltammetry , chromatography , nucleotide , gene
Electrochemical oxidation of DNA can occur at each of the four bases and guanine is the one that can suffer the easiest oxidative damage. The occurrence of the guanine oxidation product, 8‐oxoguanine, as a consequence of DNA damage caused by DNA oxidation causes important mutagenic lesions and hence it is very important to develop reliable methods for its quantification. Electrochemical study of the mechanism of oxidation of 8‐oxoguanine on glassy carbon shows that it is a reversible electrode process, pH dependent, and involves several reaction products. Electroanalytical determinations of 8‐oxoguanine were carried out and the detection limit was 8×10 –7 M.