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Electrochemical Analysis of Anthramycin: Hydrolysis, DNA‐Interactions and Quantitative Determination
Author(s) -
Teijeiro Carmen,
de la Red Emma,
Marín Dolores
Publication year - 2000
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/1521-4109(200008)12:12<963::aid-elan963>3.0.co;2-b
Subject(s) - hydrolysis , chemistry , slowness , aqueous solution , electrochemistry , detection limit , cyclic voltammetry , voltammetry , ether , dna , combinatorial chemistry , chromatography , electrode , organic chemistry , biochemistry , physics , quantum mechanics
In aqueous solution, anthramycin methyl ether (AME), anhydroanthramycin (an imino form) and anthramycin are in equilibrium. It is found that anhydroanthramycin is electrochemically active. The AME hydrolysis reaction is studied by cyclic voltammetry. The kinetics of the DNA‐drug reaction is followed by AdTSV and it is found that the slowness of the interaction is due to anthramycin changes which take place in solution independently of the DNA presence. DPP is used as a sensitive method for the quantitative determination of the drug and a detection limit of 70 nM is obtained.